Route 3 starts off with a short simple path to the north after exiting Striaton City. Straight ahead is the Pokémon Day Care and a school where several Preschoolers can be fought in the playground. A Nursery Aide can heal the player's Pokémon inside the school building. A pair of Twins initially blocks the player's path further westwards unless they have at least two usable Pokémon in their party. The player can travel westwards to find two separate paths, one that leads west and another that leads south; a patch of tall grass is located at the top of the intersection. The player can continue down the west path to find Wellspring Cave and a large patch of grass, or to the south, which winds around the shore of a pond. The player can then cross the pond by a bridge to the west leading to Nacrene City, past a few patches of tall grass. In Black and White, this is the first area where dark grass is accessible.
When the player first locates the Pokémon Day Care in Black and White, only one Pokémon can be deposited. After meeting the Day-Care Man in Nimbasa City and helping him against some Team Plasma Grunts, he will return to the Day Care and two Pokémon can now be deposited in the Day Care, allowing for Pokémon breeding to occur. In Pokémon Black 2 and White 2, this is not necessary, as the Day Care will accept two Pokémon as soon as the player reaches it.
After obtaining HM03 (Surf), the player can travel across the pond to the other side. On the west side of the pond, the player can find two patches of tall grass and a Pokémon Breeder.
A small city is also located here, as seen in Saving Darmanitan From the Bell!. The town is famous for its historic clock tower which was initially slated for demolition after its bell stopped ringing. A spate of food thefts by two Darumaka was also plaguing the town. Though thanks to Ash and his friends, the bell's hook was repaired, so the clock tower was reinstated again. Also the resident Darmanitan reverted to Standard Mode, after it was using all its energy to support the broken bell, and assumed the role of the clock tower's guardian.
A joint Pokémon Day Care and Kindergarten, operated by Karena and her granddaughter Daniela, was featured in Here Comes the Trubbish Squad!. The students had befriended a Trubbish, who brought its bad smell and the garbage from the local dump to the Day Care. Daniela was against Trubbish staying, but after it saved the children, she allowed it to stay. Karena also gifted Ash an Egg as thanks, which later hatched into a Scraggy.
The in-game maps show Route 3 leaving Striaton City directly from the west. However, the actual entrance to the city is a north-south entrance.
The two westernmost columns of tall grass in the patch closest to Nacrene City are subject to a graphical glitch: Seven tall grass sprites in each column are squashed to fit into six spaces on the world's grid. The player still moves along this grid, thus disaligning them with the grass sprites in these rows.