In these games, the Bicycle has two different gears: third and fourth gear. The player can switch between them by pressing the B button. Fourth gear is faster and allows the player to ride up muddy slopes and jump two tiles from bike ramps. Third gear is slower and only jumps one tile from ramps, but cannot ride up muddy slopes.
In Pokémon Brilliant Diamond and Shining Pearl, the player can choose between a red, blue, green, or yellow bicycle. Additionally, the player will wear the Cycling Style outfit while riding the bicycle and battling at the Cycling Road.
In Pokémon Diamond and Pearl only, bikes cannot be ridden in snow or inside gates.
The player can move in any direction using the Nintendo 3DS's Circle Pad. The player has a choice between a green and yellow Bicycle when they obtain it. They cannot switch bikes once they pick one, but both Bicycles are functionally identical.
Due to a glitch, if the player saves at the Cycling Road and creates a new game, it will not be possible to use the Bicycle (or Surf outside of battle) anywhere in the new game until the player does any of these things for the first time: blacks out (either in battle or the overworld), uses Dig, Teleport, or Fly outside of battle, uses an Escape Rope, or enters and leaves the Cycling Road.
In Generation VI, the player initially needs to switch models at Rydel's Cycles as well. However, the player will be able to keep both the Mach Bike and Acro Bike together in their Bag, after the player advertises the Rydel's Cycles to three NPCs:
In Pokémon Sword and Shield, the player receives the Rotom Bike. The Rotom Bike can occasionally dash to move at high speeds for short periods of time. After being upgraded with Water Mode, it can also travel on water.
Bicycles are rarely seen in the anime, since Ash and his friends are usually seen walking for their journeys. The only time bicycles are used is when it is necessary to use them, such as on Cycling Roads, or for a faster way to get somewhere.
Like all key items from Pokémon Ruby and Sapphire, the Mach Bike and Acro Bike are present in the internal data of Pokémon FireRed and LeafGreen, but are unused. If the player obtains one (i.e. via cheating), they both function like the regular Bicycle.
Like all key items from Pokémon FireRed and LeafGreen, the regular Bicycle is present in the internal data of Pokémon Emerald, but is unused. If attempted to be used, it has no effect.
In Pokémon FireRed and LeafGreen, when the player attempts to enter the Cycling Road, the game does not check if the Bicycle is present in the Bag; instead, it specifically checks for the event flag indicating that player has obtained the Bicycle from Miracle Cycle. However, this makes no difference in regular gameplay, because the Bicycle (like any other Key Item in these games) is unable to be deposited in the PC, sold, or thrown away, so it remains indefinitely in the Bag after the player has obtained it.
In Generation V, if the player runs or holds down the B Button, NPCs with bicycles will go faster.
In Pokémon Brilliant Diamond and Shining Pearl, the player's Cycling Style outfit will only appear in battle if they're battling on the Cycling Road. In other locations, they will appear in their Everyday Style clothes even if they were riding a bicycle before initiating a battle.
In the end credits of Pokémon Brilliant Diamond and Shining Pearl, the player always rides a green (if boy) or yellow (if girl) bicycle, regardless of the color they chose.