Upon entering the Lab with a Genesect in the party, a Scientist named Dudley will appear and become surprised that the player has the Pokémon of the canceled project that he was working on. He will request a battle with the player, then hand over two Drives (depending on game version) after being defeated.
P2 Laboratory is a small building that appears to have been unused for years; however, it contains some working machines which are still running and there are books and papers lying around. There is an item, a Dubious Disc, lying on the floor in the middle of the room. If the left side the desk is checked, a memo detailing the experiment conducted in the lab is found; it describes how the resident was hired by Team Plasma to create the strongest Pokémon but was cut off by N. The memo abruptly ends before the identity of Genesect is revealed. Outside of the lab, there are a few trees behind the building and a field with a small patch of tall grass, inhabited by wild Pokémon. In Black and White, there is also a TM on the ground and a Scientist in the field.
With the exception of Scientist Nathan, who is outside searching for Pokémon in the grass in Black and White, the P2 Laboratory is completely empty until Genesect is brought here. It is then occupied by a lone Scientist who left Team Plasma in resentment over N's decision to cancel his project, which involved reviving and enhancing Genesect.
P2 Laboratory appeared in The Name's N!. In the beginning of the episode, N was seen investigating it, leading to his discovery of data related to Genesect. Later, Cilan pointed it out as Ash and his friends passed it on a ship.
Unlike in the games, the P2 Laboratory is completely destroyed beyond function and abandoned in the anime.
P2 Laboratory was first featured in The Shadow Triad, where the Shadow Triad was seen conducting experiments at the laboratory with a mysterious Pokémon. Soon, they were attacked by Cilan, Chili, and Cress, who had followed them there. However, even with their Pokémon at a type advantage and evolved with Evolution stones to make them stronger, the Leaders of the Striaton Gym started to lose the battle. The Swords of Justice then arrived, observing the battle. Keldeo, who was able to sense the feelings of the fighting Pokémon, decided that the Gym Leaders needed to be helped. With the help of Cobalion, Virizion, and Terrakion, the Shadow Triad and their Pokémon were defeated. Not wanting the word of the intruders escaping getting out, the Shadow Triad unleashed the mysterious Pokémon at the Swords of Justice. Cobalion, Virizion, and Terrakion protected Keldeo from the Pokémon's attack, but were frozen in the process, and sunk into the depths of the sea below.
In Flying Type, Blake revealed that he had come across P2 Laboratory during his search for Team Plasma. From there, he had discovered information regarding Team Plasma's secret weapon; Genesect.
P2 Laboratory's name is similar to the Propaganda Due, known as P2 Lodge, a secret Masonic lodge in Italy that is implicated in numerous Italian crimes and mysteries. In addition, P2 reads "pedos" in Spanish, a slang for "farts". Due to this, the Italian and Spanish versions of the game renamed the laboratory to "Laboratorio P&P".