Prior to the start of the manga, Red owned Poli as a Poliwag. While swimming one day Red began to drown and Poli was unable to do anything but watch. Due to being unable to help Red in his current state he evolved into Poliwhirl and saved him. Poli first appeared in A Glimpse of the Glow, helping to capture a Nidorino for Red. Poli later battled a wild Mew, but was knocked out in a hit.
In Onix Is On!, Poli, Saur, and Pika helped Red in his Gym battle to win the Boulder Badge. Poli fought in Red's first battle against a Trainer's Graveler and was able to win by using one-hit knockouts and complimentary moves, despite starting off with low health.
In Danger: High Voltorb, Poli was the only member of Red's team to infiltrate the S.S. Anne. Immediately upon entering the two of them end up battling a Voltorb, with Poli ending up paralyzed. Due to Poli being paralyzed and unable to help out Red is forced to dodge the powerful Thunder Punch attacks from Lt. Surge's Electabuzz. Right when it seems the two of them are trapped Red reveals their plan, which had Poli secretly drip a trail of water on the floor leading to Lt. Surge and Electabuzz. At Red's command Poli uses Ice Beam to freeze the legs of Lt. Surge and Electabuzz, and with the smoke of a launched Poké Ball the two escape safely. While trying to find the exit Poli is attacked by a Magneton and captured by Lt. Surge, who throws him off the ship. Red attempts to rescue him, but is electrocuted and throw into the ocean as well. As Red begins to recall a similar situation he is saved by Poli, who evolved into a Poliwrath. With his new found strength Poli easily out powers Electabuzz with his new Seismic Toss. In The Kindest Tentacruel, it is revealed that Poli was able to evolve without a Water Stone due to the Vermilion Harbor having traces of the four Evolution stones speculated to be at the bottom of Vermilion Harbor.
In A Tale of Ninetales, Poli is accidentally traded to Blue alongside Saur and Pika. During the three days that Blue has them he trains them vigorously. They later traded back, while keeping the serious attitude that Blue installed in them.
In Meanwhile…Vileplume!, Poli battled in Red's Gym battle against Erika in his fight to save Eevee. Poli goes up against her Bellsprout, with Red being confident that it will be an easy victory. Poli attacks with Double Slap but is stopped on the last hit and counter attacked with Bellsprout's Mimic, knocking Poli out.
In Golly, Golem!, Poli is picked as Red's first Pokémon in his battle against Giovanni. Red send out Poli to attack, but in the short amount of time that he does so, Giovanni successfully releases his Cloyster and freezes Poli.
During the Pokémon League, Poli battled a Trainer's Kabutops, advancing Red to the semi-finals. In A Charizard…and a Champion, Poli and Pika helped Saur defeat Blue's Charizard in a brilliant strategy by having Poli's water vapor and Pika's electric energy create a thundercloud that struck a vine shot and wrapped around it by Saur.
The Water Stone Poli used to evolve
In Punching Poliwrath, Poli is shown in a flashback fighting against Bruno's Hitmonchan. Poli was easily able to block all of Hitmonchan's attacks and strike back with Double Slap, but was taken out when Hitmonchan attacked with Thunder Punch and Fire Punch. Poli battled in the rematch against Bruno in Clefabulous Clefable, where he battles Machamp. Machamp quickly overpowers him after his belt is removed and holds Poli down. Vee manages to use the water Poli left to use Acid Armor to surprise attack Machamp, allowing him to defeat it.
In Forretress of Solitude, Poli was part of the team that Red used in his test to become Viridian City's Gym Leader, though he was not seen participating.
Poli appeared in a fantasy in Rayquaza Redemption II, when Sapphire explained to Ruby that the accomplishments of Red and his team inspired her to train to battle.
Poli battled Carr's Steelix in Put Your Beast Foot Forward. Poli sends a Focus Punch at Steelix, sending it flying. Carr tries to counter with Steelix's Iron Tail, but Poli simply catches it in his hands, using Mind Reader to predict the attack. Poli then lifts Steelix into the air by its tail and smashes it into the ground.
In Don't Doubt Deoxys, Poli is used to battle against Organism No. 2. Poli was later nearly killed along with Red's other Pokémon while defending his Trainer from Organism No. 2's Psycho Boost.
In Distant Relation Deoxys and Storming the Forretress, Poli was seen joining Red's other Pokémon, Blue's Charizard, and Chuchu to take down the ten Forretress that Carr had placed inside the Team Rocket airship. His Damp Ability combined with Organism No. 2's use of Skill Swap enabled Red to counter Carr's final exploding Forretress.
In The Final Battle VIII, Poli joined forces with Gold's Polibo while battling against Guile Hideout. After saving Emerald and his Dusclops from Jirachi's Doom Desire attack, Poli used Double Team to confuse Guile Hideout so that Polibo could get a chance to sneak closer and attack. Although Guile Hideout tried to send his Walrein and Masquerain to attack the Tadpole and Frog Pokémon, they simply threw them away and attacked Guile Hideout directly, breaking his armor.
Poli appeared in a flashback in Pleased as Punch With Parasect, where Gold and Hirō think back to when Hirō reported on Red and Gold's training on Mt. Silver. He appeared again in a flashback in All About Arceus IX, when Hirō explains that he based Proteam Omega off of Red's team after watching him train on Mt. Silver.