The course starts out next to a hot spring with Altaria. After leaving the hot springs, the NEO-ONE reaches an area with various Fossil Pokémon such as Archeops and a Monferno. After that, the course continues into a cave inside the volcano with Graveler on the walls. After passing by some rocks, the NEO-ONE enters an area with lava flowing in the ground. Slugma and Charmander can be found here. The course ends in the lava area.
In research level 2, the NEO-ONE enters a route that leads to an area with blue lava and Torkoal instead of the route with normal lava. The blue lava later leads to an area with ancient ruins and some Typhlosion, and the course ends in the middle of some ancient ruins. Starting from Level 3, this route becomes an alternate route.