Suicune first appeared in the anime in the episode Don't Touch That 'dile. When Ash, Misty and Brock were lost in a forest, Togepi and Pikachu sensed something and led the trio to a lake. Suicune was standing on a small island on the lake. At first, Ash did not understand what Pokémon it was and wanted to capture it. However, it ran away towards the direction of New Bark Town as soon as Ash made an attempt to battle it. Ash and group chased it to New Bark from where they began their Johto adventure.
Suicune appeared once again in the episode For Ho-Oh the Bells Toll!. After Team Rocket had removed a Crystal Bell from Ecruteak's Tin Tower, many Template:Type2 Pokémon invaded the city. They wrapped Jessie, James and Meowth in a web by using String Shot and blocked off the entry to the Tin Tower. The bugs even attacked Ash, Misty, Brock, Morty and Eusine when they wanted to go to the Tin Tower. Suicune appeared and calmed down the Pokémon and freed the Rocket trio and the city from the webs of the bugs. Eusine wanted to capture Suicune and sent out his Alakazam to battle it. Alakazam used a Disable but Suicune easily blocked it with a Barrier. It then used Roar to send Alakazam back into its Poké Ball and fled from the place before Eusine could even react.
Suicune's most recent appearance was in Drifloon on the Wind!. It was a friend of two young Nurse Joys named Marnie and Paige. It appeared just in time to save Ash and Paige from falling off a bridge.
