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Revival Herbs played a major role in Stopping the Rage of Legends! Part 1 and Part 2. Clay offered to accept Ash's challenge for a Gymbattle if Ash brought him some Revival Herbs. First, Ash and his friends searched for them in the Driftveil Market, but were able to find only a single, already preordered Herb, forcing them to go to Milos Island in order to find some. Unfortunately, when they arrived there, they found out that all the Revival Herbs on the island had died out due to the lack of rain. Ash and his friends met a young man named Lewis, who told them about a legend of a Tornadus, Thundurus, and Landorus. The first two had almost destroyed the island with their fight once, only to be stopped by Landorus, who had been healed from its battle injuries by a shrine maiden and her Gothorita with a Revival Herb. As a sign of gratitude, Landorus had turned the island into a fertile paradise, blooming with Revival Herbs.
After Ash and his friends had saved the Forces of Nature from being captured by Team Rocket, effectively repeating the story of the legend in the process, the three Legendary Pokémon restored the island back to its fertile state, allowing Ash to get plenty of Revival Herbs for Clay. They were delivered to the Driftveil CityGym Leader at the beginning of Battling the King of the Mines!, in which Clay kept his promise and agreed to have a Gym battle with Ash in exchange for bringing him the herbs.
In Mewtwo — Prologue to Awakening, Anna gave Oscar's Stoutland a small bottle of Revival Herb potion to be taken to Mewtwo. However, once Stoutland found Mewtwo, the Genetic Pokémon refused to take the medicine, saying it did not want any help from people.
Multiple Revival Herbs appeared in Currying Favor and Flavor!, in which Ash and Mallow collected them to be used as ingredients for the "Akala Curry". Later, they used a couple of the herbs to heal TotemLurantis and its ally Castform after Ash's trial against it. Out of curiosity, Litten also took a taste of the herbs and, like Castform, found its bitter taste unpleasant.
It allows the player to flip a coin, then if the result is heads, one of their Pokémon is cured of any Special Conditions and 6 damage counters are removed from it. Prior to Platinum, it cannot be used on Pokémon-ex.