Unlike the Nurse Joys of Kanto, Johto, Hoenn, and Sinnoh, the Joys of Unova do not use Chansey as their helpers since they are not found in Unova. Instead, they use Audino. The first Nurse Joy seen with a Audino appears in Dreams by the Yard Full!. They serve the same function as Chansey, helping Nurse Joy take care of Pokémon, including carrying them around on stretchers and helping to heal them. They wear hats that have the same color as the Nurse Joy they work for.
In A Venipede Stampede!, the Nurse Joy of Castelia City used her Audino to help calm down the Venipede that were attacking the city with Heal Pulse.
Two of Nurse Joy's Audino were among the seven Audino controlled by Team Rocket in Where Did You Go, Audino?. In the end, the two Audino joined the others in releasing a burst of elemental attacks at the villains.