Glameow was first seen in Mounting a Coordinator Assault when it approached Dawn with her missing choker around its tail. It wasn't until the next episode, however, that it showed just how strong it really was. During the Jubilife Contest, it managed to take out Ash's Aipom, Dawn's Buneary, and Jessie's borrowed Carnivine, earning Zoey the Jubilife Ribbon. Later on it was revealed that it defeated Kenny's Prinplup as well.
Zoey used Glameow again in the Wallace Cup's semifinal round against May and her Beautifly. However it lost to Beautifly, knocking Zoey out of the competition.
It also appeared in Classroom Training! where it was revealed how it was caught by Zoey. She found Glameow abandoned in a box in an alley one cold day. Her parents wouldn't let her keep it, saying she was too young to be a Pokémon Trainer. They told her to take it to a Pokémon Center, but she decided to hide it in a shed and take care of it in secret as well. Zoey and Candice both raised Glameow together until Zoey became a Trainer, at which point Glameow joined Zoey on her journey as her starter Pokémon.
In DP176 it helped Zoey to the Semi-finals alongside her Gastrodon, and in DP177 it was a key Pokémon in Zoey's victory in the Sinnoh Grand Festival final against Dawn and alongside Gallade.
