Servine was first introduced as a Snivy in Professor Juniper's lab, where he was chosen by Trip as his starter Pokémon in In The Shadow of Zekrom!. Shortly after obtaining him, Trip challenged Ash to a Pokémon battle and used Snivy against Ash's Pikachu. As Pikachu was unable to use Electric attacks due to an encounter with Zekrom, Snivy was able to defeat him easily.
Prior to Triple Leaders, Team Threats!, Snivy was shown to have already evolved into a Servine, when Ash challenged Trip to another Pokémon battle in a Battle Club in A Rival Battle for Club Champ!. He quickly defeated Ash's Pikachu again with his Cut. During the battle with Ash's Snivy, he was revealed to be male when he became infatuated with her after being hit by her Attract. As Servine was unable to attack, he was quickly defeated by her.
It is revealed by Cilan that Servine defeated Chili's Pansear in Trip's battle for the Trio Badge.
Servine appeared again in BW034 in Trip's 3-on-3 battle with Ash. He once again battled with Ash's Snivy, this time, however, he effectively stopped Snivy's Attract with a Leaf Tornado and came out victorious with one powerful Cut attack.
Servine is set to reappear in BW041.