They appeared in person in Mother of All Battles. When Ash and his friends tried to return Larvitar, the Poacher Brothers once again tried to kidnap the mother, and this timed they managed to nab her in a Pokébag capture device. The Team Rocket trio were subtly impressed by Braggo's bravado and smooth catch that they decided to join the poachers. In a ploy to make a clean getaway with Tyranitar, Braggo and his brothers recruited Jessie and teammates, even allowing them to borrow their machine. They decoy strategy later failed after Team Rocket were sent blasting off, and Chico took control of the mecha using his remote. He went on to grab Ash's Pikachu and Ranger Mason's Croconaw, as well as bundling up Mason and the group in a Pokébag for safe measure. However, Larvitar's Screech was their undoing, and Tyranitar freed herself and destroyed the stolen Team Rocket balloon. Thanks to Ranger Mason and Larvitar, the Poacher Brothers' plan was thwarted and the trio was later arrested.
Braggo is the leader, who always speaks in rhyme and orders his two brothers around. Chico is the inventor of the three, being responsible for creating all of the machines the gang uses to poach Pokémon. The third brother is Blurt, who is self-described as quiet, but he is also clumsy and tends to break things. Together the trio are brutish and overconfident.