Prior to the start of the series Nana was given to Ruby as a Poochyena as a gift from his father, Norman. One day when Ruby was playing with Sapphire a Salamence attacked them and Ruby sent out Nana, Kiki, and Rara to fend it off. The three managed to scare it away, but Ruby got injured in the process, which scared Sapphire. Because of this incident Ruby decided to never battle in front of people again. Before moving to Hoenn, Nana was used by Ruby to win multiple contests in Johto.
Nana first appeared as a Poochyena in Creeping Past Cacnea, where she was seen with Ruby on his trip to Hoenn. After running away from home Ruby runs into Professor Birch being attacked by two Mightyena. Ruby decides to battle the Mightyena, and sends out Nana and has her use Leer. The move does nothing due to Ruby caring only about appearances and the Mightyena pounce on them again, causing Ruby to fall off a cliff.
In Hanging Around With Slaking I, Nana fought alongside Kiki against Norman and his Slaking and Vigoroth. Nana fired a Hyper Beam while Kiki attacked with Iron Tail. Despite their strength, Kiki and Nana were easily swept away due to the fact that Norman knew all of Ruby's strategies, as he was the one to teach them to him.
In It All Ends Now VIII, Nana battled alongside the rest of Ruby and Sapphire's Pokémon against Maxie and Archie. Later that day, Nana is shown to have won the Master Rank of the Cool Contest, wearing a Red Scarf.
Nana is a cool and collected individual. Much like Ruby she is flashy, loves to show off her looks, and hates getting dirty. Nana is also very stubborn at times and refuses to change her mind when she sets herself to a goal. Despite Ruby's distaste towards battling Nana is a very powerful battler.