Jolteon debuted in The Battling Eevee Brothers, under the ownership of Sparky. It was targeted in one of Team Rocket's schemes.
In Get Along, Little Pokémon, Ethan uses a Jolteon as a "sheep dog" of sorts. Whenever any of Ethan's Magnemite tried to escape, Jolteon would use Thunder Wave to lure them back.
In Trouble's Brewing and Espeon, Not Included, Satsuki, one of the Kimono Sisters, owns a Jolteon. It battled Pikachu and ultimately lost after being hit with Pikachu's Thunder.
In The Eighth Wonder of the Sinnoh World!, Volkner used a Jolteon during his Gym battle with Ash. It was defeated by Infernape.
Virgil's male Jolteon debuted in Team Eevee and the Pokémon Rescue Squad!, along with an Eevee and her other evolutions at the time. He handled several Cryogonal and powered up a generator. He reappeared in a flashback in the next episode.
In Eevee & Friends, an affectionate Jolteon was with an Eevee and its other evolutions, including the newly introduced Sylveon. It tends to shock those it hugs.
In Mystery on a Deserted Island!, one of three Eevee evolved into a Jolteon with a Thunder Stone found on the Deserted Island.
In Turning Heads and Training Hard!, a wild Jolteon was summoned to help Ilima's Eevee perform Extreme Evoboost. In the Poké Problem segment of the next episode, it saw Ilima and Eevee off.
In Out of Their Elements!, circus performer Honoka attempted to evolve her female Eevee, Curry, into Flareon, but she ended up accidentally evolving into Jolteon instead. She reappeared in a flashback in JN105.
In The Ultimate Test, Ash battled a Jolteon during the Pokémon League entrance exam.
In Pikachu Re-Volts, a Jolteon was controlled by Cassidy and Butch via their Drowzee at Mandarin Island North.
A Jolteon appeared in the Japanese credits of Pikachu's Rescue Adventure.
In As Clear As Crystal, a Jolteon was affected by a crystal that gave a lake the ability to heal Electric-type Pokémon.
In Johto Photo Finish, Gary's demonstration featured a Jolteon evolving from an Eevee.
In A Bite to Remember, Brock's demonstration featured a Jolteon evolving from an Eevee.
Multiple Jolteon appeared in the opening sequence of Destiny Deoxys.
In the opening sequence of Giratina and the Sky Warrior, a Jolteon was together with an Eevee and the other six Eeveelutions at the time.
A Trainer's Jolteon appeared in a flashback in Flint Sparks the Fire!.
A Jolteon appeared in A Campus Reunion!.
In Tag Team Battle Inspiration!, Serena's fantasy featured a Jolteon as she imagined what her Eevee could evolve into.
In a flashback in Volcanion and the Mechanical Marvel, Nikola owned a Jolteon.
A Trainer's Jolteon appeared in I Choose You!.
In The Power of Us, a Jolteon could be caught during the Pokémon Catch Race.
In Legend? Go! Friends? Go!, a Trainer's Jolteon participated in a Raid Battle against a Lugia. In To Train, or Not to Train!, it was seen at the Eevee Evolution Lab.
A Jolteon appeared in a fantasy in On Land, In the Sea, and to the Future!.
||Jolteon, the Lightning Pokémon. Jolteon becomes enveloped in lightning when angered.
In File 2: Cubone, Red's Jolteon was first sent out to battle a Team Rocket Grunt's Koffing. It eventually defeated the Poison Gas Pokémon with a Thunderbolt, forcing the Grunts to retreat. In File 3: Giovanni, Jolteon battled Giovanni's Rhyhorn during Red's Gym battle. In File 4: Charizard, Jolteon was used at the Indigo Plateau.
A Jolteon appeared in The Show, under the ownership of Sayo. It was used in the Kimono Girls' performance of the destruction of the Brass Tower, where it represented the lightning that set the tower on fire.
Another Jolteon appeared in The Discovery, under the ownership of Trace. He used it to fend off a swarm of Zubat in Cerulean Cave.
The Electric Tale of Pikachu
A Jolteon belonging to Sparky appeared in To Evolve or Not to Evolve, That Is the Question!, where it was used to battle Mikey's Eevee in order to see if Mikey was worthy of joining the Knights of the E Stone. Thanks to Ash using a TM to teach Eevee Mimic, it was able to defeat Jolteon by using its own Pin Missile against it, winning the battle for Mikey.
- Main article: Vee
In Blame it on Eevee, a mutated Eevee could switch between his evolved forms, one of which is a Jolteon. Red caught him, added him to his team, and nicknamed him Vee. Later, it used the Fire, Water, and Thunder Stones of Vermilion Harbor, which were given to him by Giovanni, to evolve and devolve him freely and faster.
A Jolteon appeared in The Kindest Tentacruel as one of the Pokémon in Yellow's fantasy about evolution via Evolution stone.
A Jolteon appeared in Pleased as Punch With Parasect, under the ownership of a Kimono Girl.
A Jolteon appeared in a fantasy in Sylveon Enchants.
Pokémon Pocket Monsters
A Jolteon appeared in Regain the Deserted Power Plant!.
A Jolteon appeared in Clefairy Evolves... At Last?!.
A Jolteon appeared in Curry Showdown! Which is the Most Delicious? as one of the participants of a curry cooking competition.
Giovanni sent out a Jolteon when he was about to destroy Pallet Town with a missile in Squirtle, the Crybaby Pokémon. It reappeared in Good-Bye, Pikachu?!.
A Jolteon appeared in a fantasy in PZ04.