In Jessilina's Sinnoh Contest debut, Carnivine was used in both the Performance Stage and the Battle Stage. In the first round, Carnivine used Bullet Seed on a lollipop that Jessie threw, and amazed the crowd by biting Jessie's head. The performance impressed the Contest Judges and, as a result, Jessilina was one of the eight Coordinators moving on to the second round. There, it was seen defeating a Rhydon, showing that it was a skilled battler, and thanks to it, Jessie reached the final stage. However, it lost against Zoey's Glameow.
Carnivine was also used in Dressed for Jess Success! to compete in the Battle Stage of the Lilypad Contest. Together with James, who was the one disguised as Jessilina at that time, they defeated Dawn and her Mamoswine in the semifinals, and eventually won the Ribbon.
In Yes in Dee Dee, It's Dawn!, Carnivine teamed up with Seviper to perform at the Daybreak Town Pokémon Contest, which was being held under the Double Performance rule. It walked into the stage alongside Jessilina with Seviper's Poké Ball inside its mouth. When Jessilina began playing her Arbok-shaped flute, Seviper popped out of its Poké Ball and joined Carnivine. The two were able to dazzle the judges and the audience with their performance, which secured Jessilina a spot in the Battle Stage.
Carnivine made its final appearance in Memories are Made of Bliss!, where it battled Ash and his friends for the final time, but got knocked back by Togekiss's Air Slash before getting blasted off with the rest of the team.
Following the Team Rocket trio's journey in Sinnoh, James was forced to leave Carnivine at Team Rocket's headquarters, as it is not a Pokémon native to the Unova region and would seem suspicious.
Personality and characteristics
Carnivine showing affection
Carnivine continues the series' running gag of James's Grass-type Pokémon showing affection for him by hurting him whenever they are sent out from their Poké Balls. This affection is probably due to the fact that James and Carnivine shared a close bond when James was a child.
Carnivine shares a great relationship with James, like his other Pokémon. In Saving the World From Ruins!, Carnivine saw the fear in James's eyes at the face of death and heroically learned Vine Whip to rescue him and the others.