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Amber (Japanese: アイ I) was the daughter of Dr. Fuji, who died while she was still a child. She first appeared in The Birth of Mewtwo radio drama, released in CD format exclusively in Japan, and then in The Uncut Story of Mewtwo's Origin short, which was added to the beginning of Mewtwo Strikes Back in all television airings in Japan and in the Japanese home video releases from the third one onward.
Dr. Fuji's despair at losing his daughter led him to dedicate most of his life to research into cloning, with the belief that he would be able to create a clone of Amber if he discovered the secrets of recreating life. This led Giovanni to fund him, with the condition that he would also clone Mew, creating Mewtwo.
Both Mew and Amber were successfully cloned, and Amber's clone was named Ambertwo. While they were both growing inside test tubes on New Island, Mewtwo communicated with Ambertwo telepathically. Unfortunately for Fuji, Mewtwo's genetic makeup (as a Pokémon) and sheer force of power kept him alive while Ambertwo died. Fuji was heartbroken, but continued his work on Mewtwo with the hope that another try at cloning Amber would be more successful.
However, the last incarnation of Amber died as Mewtwo destroyed the laboratory on New Island, as well as any hopes of seeing her alive again.
- In the early stages of the radio drama's production, Amber was originally referred to as Me, but was later renamed to I as the writer felt that it connotes a stronger assertion of self-existence. The name Me was later used on a different character.
- If one counts the radio drama and The Uncut Story of Mewtwo's Origin, Amber is the first human to be seen dying in the Pokémon anime.