In Losing Its Lustrous!, Professor Carolina of the Celestic Town Historical Research Center was studying the Lustrous Orb, and was having the Adamant Orb transported there to be studied as well. Upon receiving word that the Adamant Orb had been stolen by Team Galactic, the Lustrous Orb was put under heavy police protection by Officer Jenny and her officers. Professor Carolina later showed Ash, his friends, Cynthia, and Cyrus the Orb's hidden away location for further examination. However, after Mars set off some explosives outside of the building, the door to the Orb room failed to reseal. While the group attempted to move the Lustrous Orb to another location, Team Galactic burst in and engaged the group and Cynthia in battle.
In Double Team Turnover!, Team Rocket succeeded in stealing the Lustrous Orb while disguised as police officers. Ash, his friends and Team Galactic tried to get the Orb back, following Team Rocket to the Celestic Ruins. Cornered, Team Rocket threatened to have James's Carnivine swallow the item. Mars's Purugly suddenly jumped on Carnivine's head and grabbed the Lustrous Orb, allowing Team Galactic to get away with it. Back in the Team Galactic HQ, the Lustrous and Adamant Orbs were shown being connected to some kind of machinery.
The two Orbs made a brief appearance at the end of Unlocking the Red Chain of Events!, where Team Galactic was seen taking them to the Spear Pillar, along with the Red Chains. The Orbs reappeared in The Battle Finale of Legend!, where Cyrus placed them and the lake guardians around the ruins at the Spear Pillar. This process allowed him to call forth Dialga and Palkia, who were immediately taken under his contol by the Red Chains. After Cyrus's attempt to create a new world had been foiled and Team Galactic was defeated, Gary secured the Orbs and decided to return them to Professor Carolina and the Celestic Research Center.
In the TCG
The Lustrous Orb is featured in the Pokémon Trading Card Game as a held item on Palkia. This held item works in the same fashion as a Poké-Body, in that the effect the item provides is active whenever the Pokémon is in play. The following is a list of cards including the Lustrous Orb.
Pokémon cards with the Lustrous Orb as a held item Cards listed with a blue background are only legal to use in the current Expanded format. Cards listed with a silver background are legal to use in both the current Standard and Expanded formats.