Generation III
A Tamato Berry will mature from a planted seed to a full-grown, fruit-bearing tree in 24 hours, with 6 hours per stage. A Tamato tree will yield 2-4 Berries.
Generation IV
A Tamato Berry will mature from a planted seed to a full-grown, fruit-bearing tree in 32 hours, with 8 hours per stage. A Tamato tree will yield 1-5 Berries.
Whenever an episode features Berries, such as The Lotad Lowdown, or Berry, Berry Interesting, a character may come across this berry and decide to eat it. They instead experience its intense spiciness, and may end up with swollen lips for a while.
In Shocks and Bonds Brock says that an Ace Trainer named Katie who battled Ash in the Hoenn League likes Tamato Berries in her Spaghetti.
In Cooking Up a Sweet Story!, some Tamato Berries were seen in Abigail's Berry basket
In Double-Time Battle Training!, Candice states that a treat called the "Snowpoint Tamato Berry Ice Cream Bar" has cold Ice Cream outside and spicy Tamato Berry Jelly on the inside, which Ash accidentally ate whole and which Dawn based her Daybreak Town Appeals on.
A Tamato Berry plant appeared in PK23. Piplup showed Meloetta, Pikachu, and Snivy the plant in their search for a Rinka Berry, certain that it was a Rinka plant. When they told Piplup it was the wrong plant, Piplup stubbornly ate a Tamato Berry, only to find it was too spicy. Meowth and Wobbuffet, who were following Meloetta, found the Berries and decided to eat them, only to be overwhelmed by its spiciness.